SupelMIP™ Chloramphenicol
- A selective SPE sorbent optimised for extraction of the banned antibiotic Chloramphenicol in biological matrices.Background
Chloramphenicol is a broad-spectrum antibiotic frequently employed in animal and fish production in certain parts of the world because of its excellent antibacterial and pharmacokinetic properties. Due to the suspicion that Chloramphenicol is carcinogenic and that it can cause aplastic anaemia in humans it was totally banned in the EU in 1994. Today, a zero tolerance level for food exists since maximum tolerance levels cannot be established for compounds with this level of toxicity.
Features and applications
SupelMIP™ Chloramphenicol extracts the broad spectrum antibiotic Chloramphenicol selectively from biological matrices with high and reproducible recoveries (over 80% from milk), allowing sharp decision limits. The estimated detection limit of 0.01 µg/kg is well below the minimum required performance limit (MRPL) for Chloramphenicol, which is set at 0.3 µg/kg. The product is delivered with a data sheet optimized for extraction of Chloramphenicol from milk, honey and urine samples, with MS detection. Skimmed milk can be loaded directly on the column. This robust and highly selective SupelMIP™ Chloramphenicol is, of course, well suited for use in high-throughput automated systems.
More information about SupelMIP™ Chloramphenicol can be found in the Product Bulletin. Recovery is given together with analytical method, chromatograms and SPE procedure in the Data Sheet.
| Code | Sorbent mass | Column volume | Columns/box | Order |
| 53209-U | 25 mg | 3 ml | 50 |  www.sigmaaldrich.com |
| 53210-U | 25 mg | 10 ml | 50 | www.sigmaaldrich.com |
Literature
- Development of an improved method for trace analysis of chloramphenicol using molecularly imprinted polymers. Boyd B, Björk H, Billing J, Shimelis O, Axelsson S, Leonora M, Yilmaz E, J Chromatogr A. 2007 Dec 7;1174(1-2):63-71.
- The selective extraction of Chloramphenicol using molecular imprinted polymer (MIP) SPE
The Reporter vol 25.2 - Advantages of Molecularly Imprinted Polymers LC-ESI-MS/MS for the Selective Extraction and Quantification of Chloramphenicol in Milk-Based Matrixes. Comparison with a Classical Sample Preparation. Rayane Mohamed, Janique Richoz-Payot, Eric Gremaud, Pascal Mottier, Ecevit Yilmaz, Jean-Claude Tabet,§ and Philippe A. Guy*, Nestlé Research Center, Nestec Limited, P.O. Box 44, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland, MIP Technologies, Scheelevägen 22, Box 737, 22007 Lund, Sweden, and § Laboratoire de Chimie Structurale Organique et Biologique, CNRS UMR 7613, University Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris cedex 05, France. Anal Chem. 2007 Dec 15;79(24):9557-65. Epub 2007 Nov 15.
