SupelMIP™ Chloramphenicol

- A selective SPE sorbent optimised for extraction of the banned antibiotic Chloramphenicol in biological matrices.

Background

Chloramphenicol is a broad-spectrum antibiotic frequently employed in animal and fish production in certain parts of the world because of its excellent antibacterial and pharmacokinetic properties. Due to the suspicion that Chloramphenicol is carcinogenic and that it can cause aplastic anaemia in humans it was totally banned in the EU in 1994. Today, a zero tolerance level for food exists since maximum tolerance levels cannot be established for compounds with this level of toxicity.

Features and applications

SupelMIP™ Chloramphenicol extracts the broad spectrum antibiotic Chloramphenicol selectively from biological matrices with high and reproducible recoveries (over 80% from milk), allowing sharp decision limits. The estimated detection limit of 0.01 µg/kg is well below the minimum required performance limit (MRPL) for Chloramphenicol, which is set at 0.3 µg/kg. The product is delivered with a data sheet optimized for extraction of Chloramphenicol from milk, honey and urine samples, with MS detection. Skimmed milk can be loaded directly on the column. This robust and highly selective SupelMIP™ Chloramphenicol is, of course, well suited for use in high-throughput automated systems.

More information about SupelMIP™ Chloramphenicol can be found in the Product Bulletin. Recovery is given together with analytical method, chromatograms and SPE procedure in the Data Sheet.

Code	Sorbent mass	Column volume	Columns/box	Order
53209-U	25 mg	3 ml	50	www.sigmaaldrich.com
53210-U	25 mg	10 ml	50	www.sigmaaldrich.com

Literature and informative material

Publications

Determination of chloramphenicol in urine, feed water, milk and honey samples using molecular imprinted polymer clean-up, Rejtharová M and Rejthar L, Journal of Chromatography A, (2009), 1216 (46), 8246-8253
Development of an improved method for trace analysis of chloramphenicol using molecularly imprinted polymers, Boyd, B, Björk H, Billing J, Shimelis O, Axelsson S, Leonora M, Yilmaz E, Journal of Chromatography A (2007), 1174(1-2):63-71
Advantages of molecularly imprinted polymers LC-ESI-MS/MS for the selective extraction and quantification of Chloramphenicol in milk-based matrices. Comparison between a classical sample preparation., Mohamed R, Richoz-Payot J, Gremaud E, Mottier P, Yilmaz E, Tabet JC and Guy P, Anal.Chem. (2007), 79(24), 9557-9565

Data Sheet