SupelMIP™ Clenbuterol

- A specific SPE sorbent optimised for extraction
of clenbuterol from biological samples.

Features and applications

Clenbuterol is extracted with a high recovery. Due to the selectivity this extraction method enables determination at a limit of quantification of 0.5 ng/ml using the economically favourable detection system: HPLC-UV.

The chromatograms show the extraction clean-up of a typical analyte from a 5 ml urine sample. Superior clean-up with the MIP sorbent is shown in red, whereas the other chromatograms show clean-up with a much higher level of chemical noise, using mixed-phase SPE sorbents.

More information about SupelMIP™ Clenbuterol can be found in the Product Bulletin.
Recovery, precision and accuracy are given together with chromatograms and SPE procedure in the Data Sheet. For further reading we also refer to the literature list below.

Code	Sorbent mass	Column volume	Columns/box	Order
53201-U	25 mg	10 ml	50	www.sigmaaldrich.com

Literature

• Extraction of clenbuterol from calf urine using a molecularly imprinted polymer followed by quantification by high-performance liquid chromatography with UV detection. PMID:12458756.
  J Chromatogr A.2002 Oct 25;975(1):157-64.
• Recent reports have suggested that abuse of the beta agonist Clenbuterol in meat production has been the main reason for collective intoxication outbreaks in humans, particularly in China, Mexico and Portugal. Abuse of clenbuterol and its detection. Prezelj A, Obreza A,Pecar S
• Molecular imprinted polymer SPE increases sensitivity for the extraction and analysis of clenbuterol from urine
   The Reporter vol 25.2