Can Betanin be Separated with Only Aqueous Solvents?


The resin RENSA® PY is capable of fractionating the various dyes in beetroot juice with a purely aqueous elution regime without the use of salt or organic solvent gradients. This is demonstrated in a new proof-of-concept study from Biotage.

Application Note – Fractionation of Betanin Food Dyes on RENSA® PY »

Food colorants are important ingredients in food and beverage, and also pharmaceutical and cosmetic products. The trend within these industries is strongly moving away from artificial towards natural dyes originating preferably from plants, beetroot being one important source. The most important and most studied color compound in beetroot is betanin, also called beetroot red (E number E162).

This proof-of-concept investigation demonstrates that the resin RENSA® PY can separate betanin in an aqueous beetroot juice, and fractionate the dye compounds with the potential to retrieve a targeted fraction of a certain dye type. The process conditions are mild and neither salt gradients nor organic solvents are required for the dye separation.

Other resin types used for betanin retention such as reversed phase resins or ion exchangers typically do not display sufficient retention of the highly polar betanin, and require organic solvents or high amounts of salts to elute off bound betanin. The presented approach provides clean betanin fractions without organic solvents or high salt concentrations, vital factors for an economically feasible process implementation of this resin.

RENSA® PY is developed by MIP Technologies, a subsidiary of Biotage specializing in the design and scale-up of tailored resins for purification of targeted compounds.