SupelMIP™ Nitroimidazole
- A Class-selective SPE sorbent for extraction of nitroimidazoles from food samples e.g. milk and egg samplesBackground
Nitroimidazoles are a group of broad-spectrum antibacterial and anticoccidial drugs used for treatment of cattle, poultry, pigs and farmed fish. These compounds and their metabolites are suspected to be carcinogens and mutagens. Consequently, several Nitroimidazoles are classified in the European Union (EU) list of pharmacologically active substances for which no maximum residue limit can be fixed, meaning that their use is forbidden in food-producing animals. Some compounds in the class have never been authorized as a veterinary drug and therefore is also considered as a forbidden. Monitoring these substance residues at trace levels is therefore regulated by law. The surveillance of nitroimidazole drug residues requires the detection of these compounds as well as their main metabolites at trace levels in animal products.
Features and applications
The SupelMIP™ Nitroimidazoles is a simple, fast, selective and sensitive SPE product for selective extraction of Nitroimidazole residues from complex matrices.
The SPE phase has been developed for multi-residue extraction of nitroimidazoles and has been validated for Dimetridazole (DMZ), ipronidazole (IPZ), metronidazole (MNZ), and ronidazole (RNZ) and their hydroxylated metabolites (DMZOH and MNZOH). Using the specially developed SupelMIP SPE; nitroimidazoles were extracted from milk and egg samples (fresh eggs and egg powder) with high and reproducible recoveries with low RSD.
A data sheet with general guidelines for this SPE is available. Specific applications for extraction of nitroimidazoles from milk and egg samples with LC-MS/MS detection is available.
| Code | Sorbent mass | Column volume | Columns/box | Order |
| 52734-U | 50 mg | 3 ml | 50 |  www.sigmaaldrich.com |
Literature and informative materials
Publications
- Use of Molecularly Imprinted Solid-Phase Extraction Sorbent for the Determination of Four 5-Nitroimidazoles and Three of Their Metabolites from Egg-Based Samples before Tandem LC-ESIMS/MS Analysis. R.Mohamed, P.Mottier, L.Treguier, J.Richoz-Payot, E.Yilmaz, J-C.Tabet, AND P.A.Guy*, Nestlé Research Center, Nestec Ltd., P.O. Box 44, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland, MIP Technologies, Lund, Sweden, and Laboratoire de Chimie Structurale Organique et Biologique, University Pierre & Marie Curie, France. J. Agric. Food Chem. 2008, 56, 3500-3508
Application note
- SupelMIP SPE - Nitroimidazoles Instruction Sheet (id. T709075)
Supelco Reporter articles and technical notes etc
Press releases
