SupelMIP™ NNAL

- A selective SPE sorbent optimised for extraction of tobacco-specific nitrosamine NNAL in biological samples.

Background

NNAL is a cancerogenic compound originating from nicotine metabolites and is an important marker in the monitoring of the effects of such compounds in humans. NNAL is a metabolite of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

Features and applications

SupelMIP™ NNAL extracts 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) selectively from biological samples with high recoveries. Compared to current analytical methods for accurate NNAL detection, which are elaborate and time-consuming, SupelMIP™ NNAL gives reliable and accurate results in just a fraction of the time. It allows extremely low limits of quantitation and has been shown to be very reproducible. It can also be used for quantitation of NNAL where nicotine is present. This robust and highly selective SupelMIP™ NNAL is, of course, well suited for use in high-throughput automated systems.

More information about SupelMIP™ NNAL can be found in the Product Bulletin
Recovery, precision and accuracy are given together with chromatograms and SPE procedure in the Data Sheet. For further reading we also refer to the literature list below.

Code	Sorbent mass	Column volume	Columns/box	Order
53203-U	25 mg	3 ml	50	www.sigmaaldrich.com
53206-U	25 mg	10 ml	50	www.sigmaaldrich.com

Literature

• Analysis of the Tobacco-Specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in urine by extraction on a molecularly imprinted polymer column and liquid chromatography/atmospheric pressure ionization tandem mass spectrometry., Xia Y, McGuffey J, Bhattacharyya S, Sellergren B, Yilmaz E, Wang L., Bernert J.
   Anal. Chem., 2005, 7639-7645.
• Analysis of the Tobacco-specific Nitrosamine 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol in Urine by SPE on a Molecularly Imprinted Polymer (MIP) Column and LC MS/MS, Xia Y, McGuffey J, Wang L, Bernert J, Centers for Disease Control and Prevention, Atlanta, USA
   Poster
• Trace level analysis of NNAL in urine using supelMIP™ SPE - NNAL and LC-MS-MS
   Poster