SupelMIP™ PAH
- A selective SPE sorbent for extraction of high cycle PAHs from organic matrices.Background
Polyaromatic hydrocarbons (PAHs), many of which are carcinogens, consist of fused aromatic rings and are formed as by-products during combustion. Seven PAHs in particular are known for their carcinogenic properties: benz[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, chrysene, dibenz[a,h]anthracene, and indeno[1,2,3-cd]pyrene. As a result, they are closely monitored in both the environment and food.
One of the most potent PAH's, benzo[a]pyrene, is found in a number of edible oils (olive, grapeseed, pomace, etc.) and is generated during the production process of such oils. For example, the use of contaminated solvents during olive mash extraction and extensive heat treatment during solvent evaporation, have been attributed to PAH contamination.
On February 4th, 2005, the European Union Commission Regulation (EC) No 2008/2005 set maximum limits of PAHs in food. For example, in oils and fats intended for direct human consumption, the maximum residue limit, based on benzo(a)pyrene levels measured on wet weight, is 2 µg/kg
(http://www.ihta.org/word_documents/EU_sets_maximum_levels_of_PAHs_in_food.doc).
Further, new regulations on levels of PAHs in ‘extended fuels’, also governed by EU Regulation 2008/2005, come into effect Jan 1, 2010
Features and applications
An effective SPE method has been determined for PAHs and can be further optimized for a number of matrices. The PAHs that have been tested include: Fluoranthene, benzo[a]anthracene, chrysene, benzo[k]fluoranthene, benzo[b]fluoranthene, benzo(a)pyrene, indenol[1,2,3-cd]pyrene, dibenzo[a,h]anthracene and benzo[ghi]perylene. The SupelMIP PAHs is not suitable for extracting smaller PAH compounds (2-3 rings) or for quantitative extraction of pyrene.
Two procedures have been developed. The first is a general procedure using non-polar organic solvents such as cyclohexane to extract the PAHs with subsequent SPE on the SupelMIP and analysis by gc/ms. This general procedure represents a recommended starting point for further optimization.
In addition, a method specific for measurement of PAHs in olive oil is available. This procedure gives high recoveries (average recovery of 78% for 9 different PAHs) and high sensitivity 0.2 ppb for benzo(a)pyrene and 0.02 ppb for benzo[a]anthracene
| Code | Sorbent mass | Column volume | Columns/box | Order |
| 52773-U | 50 mg | 3 ml | 50 |  www.sigmaaldrich.com |
Literature and informative materials
Publications
Application note
